An electron micrograph of a thin frozen section treated with a clone of the mAb (D40-2) used to show the A4 antigen on decorated tubules. The immunogold lies along the outer margin of these tubules fo...

A live amoeba treated with the membrane-binding vital dye FM4-64 which stains the plasma membrane, contractile vacuole and other organelles. Large membrane-bounded organelles are excluded from the bro...

Quick-freeze deep-etch of the contractile vacuole E-fracture face. This face like the collecting canal membrane is porous indicating that many IMPs have been removed from this leaflet during fracturin...

A collecting canal of the contractile vacuole surrounded by smooth spongiome remnants and decorated tubules of the CVC. Microtubules bordering the flattened collecting canal are labeled with immunogol...

Contractile vacuole pore in face view illustrating the microtubular ribbons that extend from the pore to the CV membrane, the coil of microtubules that surrounds the pore and smaller pellicular pores ...

The contractile vacuole and cortex of Didinium nasutum showing an extrusive organelle (cyrtocyst) of unknown function. Further details available at Wessenberg, H. and Antipa, G. 1968. Studies on Didin...

Quick-freeze deep-etch image of a fracture through the radial canal of the contractile vacuole. This technique presents a picture of a confusing array of non-etchable material surrounding a collecting...

The two CV pores of a cell. An impression of the helically wound microtubules are detectable in the right pore. TEM taken on 4/21/78 by R. Allen with Hitachi HU11A operating at 60kV. Neg. 21,750X. Bar...

A circular depression on the dorsal surface of the cell marks the expulsion site of a contractile vacuole. This is referred to as the contractile vacuole “pore.". Microtubules encircle the pore. Tr...

Contractile vacuole in the opisthe of a dividing cell. This is presumably the mature CV of the original “mother” cell. Spongiome tubules form large regions of additional membrane on two sides of t...