CIL:15713, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Didinium nasutum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:contractile vacuole, cell cortex, cytoplasm, , ; Biological process:contractile vacuole organization, cortical cytoskeleton organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:15713^* Cite

Description

The contractile vacuole and cortex of Didinium nasutum showing an extrusive organelle (cyrtocyst) of unknown function. Further details available at Wessenberg, H. and Antipa, G. 1968. Studies on Didinium nasutum. I. Structure and ultrastructure. Protistologica 4:427-447. TEM taken in 1966 by G. Antipa with JEM T6S operating at 50kV. Neg. 6,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 90nm thickness.

Biological Sources

NCBI Organism Classification: Didinium nasutum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Carolina Biological Supply Company, NC, U.S.A.
Cellular Component: contractile vacuole; cell cortex; cytoplasm; extrusome; cyrtocyst

Biological Context

Biological Process: contractile vacuole organization; cortical cytoskeleton organization

Attribution

Names: Gregory Antipa (San Francisco State University)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL15713
Archival Resource Key (ARK): ark:/b7295/w9cil15713

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4828px	1.7nm
Y	6304px	1.7nm