CIL:21716, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:contractile vacuole, contractile vacuolar membrane; Biological process:contractile vacuole organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:21716^* Cite

Description

Quick-freeze deep-etch of the contractile vacuole E-fracture face. This face like the collecting canal membrane is porous indicating that many IMPs have been removed from this leaflet during fracturing. The microtubular ribbon seen here is composed of at least 24 microtubules and is divided into 3 sub-ribbons. Only a few IMPs remain in this leaflet. TEM taken on 6/22/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 24,800X. Bar = 0.2µm.A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:13127) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: contractile vacuole; contractile vacuolar membrane

Biological Context

Biological Process: contractile vacuole organization

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL21716
Archival Resource Key (ARK): ark:/b7295/w9cil21716

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1967px	——
Y	2382px	——