Figures 264 (upper) and 265 (lower) from Chapter 8 (Lysosomes) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Lysosomes apparent in thin sections from liver tissue of hamster treated with phenobarbito...

In order to study all stages of DVs in the same cell we used a protocol of sequentially exposing cells to different sizes of latex beads interspersed with washes: for example, 3 minutes in 0.1µm bead...

Figures 272 (upper) and 273 (lower) from Chapter 8 (Lysosomes) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. Lysosomes in the basal region of epithelial cells from the cauda epididymis of the rabbit....

Secondary lysosomes contain a lumen in quick-freeze deep-etch (QF-DE) images resembling etched matrix proteins rather than the honeycomb appearance of acidosomes. Lysosomes often have one or more para...

Secondary lysosomes in quick-freeze deep-etch images lie against the smooth E-face of a phagoacidosome (DV-II) membrane. The P-fracture face of one fairly large lysosome lies close to the DV-II but no...

Secondary lysosomes contain a fairly dense matrix in well fixed cells. The content of these lysosomes seems to be released as a package into the phagoacidosome when they fuse to form the phagolysosome...

This micrograph shows a crystal surrounded with secondary lysosomes. This was taken from a cell returned to growth medium following starvation for several days (0 day of rejuvenation). This could repr...

A DV-III contains a thick continuous glycocalyx (polysaccharide coat) on the luminal side of its membrane as well as paracrystalline material, both provided by the fused lysosomes. The latex beads ind...

As with acidosome fusion with the DV-I, lysosome fusion with the DV-II also seems to occur nearly all at once resulting in a phagolysosome (DV-III) with a highly irregular surface. However, unlike the...

The carrier vesicles dock with the acidosomes and then fuse with these larger vesicles before or as the vesicles dock at the nascent Digestive Vacuole. Thus the contents of the carrier vesicles are fi...