CIL:36740, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:secondary lysosome, cytoplasm; Biological process:digestive system process, cytoplasm organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36740^* Cite

Description

Secondary lysosomes in quick-freeze deep-etch images lie against the smooth E-face of a phagoacidosome (DV-II) membrane. The P-fracture face of one fairly large lysosome lies close to the DV-II but no fusion pore is seen. The P-face of the lysosome has a moderate number of IMPs, more IMPs than on its E-fracture face. TEM taken on 5/25/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: secondary lysosome; cytoplasm

Biological Context

Biological Process: digestive system process; cytoplasm organization

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36740
Archival Resource Key (ARK): ark:/b7295/w9cil36740

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1212px	——
Y	1328px	——