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Center for Research in Biological Systems
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Search Results for
ethanol fixed tissue
(27 results)
CIL:187
NCBI Organism Classification
Drosophila melanogaster
Biological Process
spermatogenesis
Cellular Component
cytoplasm
The image shows a developing Drosophila spermatid immunostained for caspase activity (green). TRITC-phalloidin is used to label actin (red). Caspase is required in spermiogenesis for the elimination ...
CIL:39234
NCBI Organism Classification
Mus musculus
Biological Process
DNA replication involved in S phase
Cellular Component
chromatin
Shown is a 0.5 micrometer thick confocal image of a midplane section of a 3T3 fibroblast pulsed for 5 min with BrdU and immuno-labeled with anti-BrdU antibodies. Replication is confined to discrete si...
CIL:39236
NCBI Organism Classification
Mus musculus
Biological Process
DNA replication involved in S phase
Cellular Component
chromatin
Shown are 0.5 micrometer thick confocal image of midplane sections of synchronized 3T3 fibroblasts pulsed for 2 min with CldU and labeled with FITC (green) followed by a variable chase time, and a sec...
CIL:13868
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
nucleus
During late anaphase, Tem1 (green) localized to both spindle pole bodies (SPBs), but Bfa1 (red) was asymmetrically localized to the daughter SPB. DAPI (blue) and DIC (hidden) are also shown. Compare t...
CIL:13869
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
nucleus
Both Tem1 (green) and Bfa1 (red) are localized to both spindle pole bodies in metaphase. DAPI (blue) and DIC (hidden) are also shown. Compare to anaphase image CIL# 13868. Image is Fig 6A, top panels,...
CIL:39235
NCBI Organism Classification
Macropus sp. MG002
Biological Process
DNA replication involved in S phase
Cellular Component
nuclear chromosome
Shown is a chromosome spread of a PtK1 cell pulsed with BrdU in early S phase, then treated with colcemid to arrest cells at metaphase. Spreads were immuno-labeled with FITC-conjugated anti-BrdU antib...
CIL:13874
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
cytoskeleton
Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows a rebudded cell, CIL #1387...
CIL:13877
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
nucleus
Tem1 (green) localizes to spindle pole bodies (SPB) during metaphase. Control image for CIL# 13876 in which Tem1 loading onto SPBs was severely affected in bfa1∆ cells. DAPI (blue) and DIC (hidden) ...
CIL:13880
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
regulation of exit from mitosis
Cellular Component
spindle pole body
Tem1-CAAX (green) is targeted away from spindle pole bodies in wild-type cells (this image) but can also still localize to the SPB (CIL# 13881). The CAAX motif from Ras2 targets proteins to the cytopl...
CIL:13870
NCBI Organism Classification
Saccharomyces cerevisiae
Biological Process
cell cycle
Cellular Component
nucleus
Tem1 (green) loading onto spindle pole bodies during anaphase is severely affected in bfa1Δ cells. Compare with control image CIL# 13871. DAPI (blue) and DIC (hidden) are also shown. Image is Fig 6B,...
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