Intracellular localization of GFP-Vps74 in BY4742 vps74∆. GFP-Vps74 is localized to Golgi compartments and the cytosol. Control image for images showing ectopic expression of GFP-Vps74 with mutation...

Intracellular localization of GFP-Vps74 W88A, R97A mutant in BY4742 vps74∆. GFP-Vps74 is localized to Golgi compartments and the cytosol (CIL# 13449) but loses localization when two amino acids are ...

Kre2-GFP is not efficiently maintained in the Golgi apparatus in BY4742 vps74∆ expressing Vps74 with mutations in the sulfate-binding pocket (W88A R97A). Compare with control image CIL# 13450. This ...

COP-I beta1 (green) staining at the Golgi (GM130, a cis-Golgi marker) (red) in unstimulated HeLa cells. Helix Pomatia Lectin (HPL) (gray) staining is also shown. HPL binds various glycans but the Tn a...

Intracellular localization of GFP-GOLPH3 in a wild-type S. cerevisiae background grown at 37C. In wild-type cells incubated at 26C or 37C, GFP-GOLPH localizes to intracellular membranes more robustly ...

Constituvely active mutant Arf1-GFP (Arf1(Q71L)-GFP) (gray) blocks redistribution of Helix Pomatia Lectin (HPL) (green) from Golgi to ER in Src-deficient SYF fibroblasts cotransfected with active Src...

Intracellular localization of GFP-GOLPH3 in BY4742 sac1∆ vps74∆. SAC1 encodes an integral membrane phosphoinositide phosphatase that is localized to the ER and Golgi. In the sac1∆ vps74∆ mutan...

Intracellular localization of GFP-Vps74 K178A, R181A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-Vp...

Intracellular localization of GFP-GOLPH3 W81A, R90A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-GOL...

Intracellular localization of GFP-Vps74 in a wild-type S. cerevisiae background grown at 26C. In wild-type cells incubated at 26C or 37C, GFP-Vps74 is localized to Golgi compartments and the cytosol. ...