CIL:9812, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Opercularia [NCBITaxon:168247], , ; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:macronucleus, oral apparatus, contractile vacuole, cell cortex, cytoplasm; Biological process:macronucleus organization, cortical cytoskeleton organization, cytoplasm organization, contractile vacuole discharge;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9812^* Cite

Description

A contractile vacuole in systole. The CV has collapsed into vesicles and tubules. Three CV pores can be seen where the CV meets the pellicle. Smooth membrane cisterna surrounds some cytoplasm, possibly a developing stage of an autophagic vacuole. TEM taken on 6/12/69 by R. Allen with Philips 300 operating at 60kV. Neg. 9,250X. Bar = lµm. A print of the negative was scanned and processed in Photoshop. This image is suitable for qualitative analysis. There is a high resolution version of this image in the library (CIL:39335) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.

Biological Sources

NCBI Organism Classification: Opercularia [NCBITaxon:168247]; Opercularia coarctata
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1657/1
Cellular Component: macronucleus; oral apparatus; contractile vacuole; cell cortex; cytoplasm

Biological Context

Biological Process: macronucleus organization; cortical cytoskeleton organization; cytoplasm organization; contractile vacuole discharge

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9812
Archival Resource Key (ARK): ark:/b7295/w9cil9812

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3024px	——
Y	3782px	——