CIL:9809, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Opercularia [NCBITaxon:168247], , ; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:macronucleus, oral apparatus, mitochondrion, cell cortex, cytoplasm, contractile vacuole, micronucleus; Biological process:macronucleus organization, cortical cytoskeleton organization, cytoplasm organization, contractile vacuole organization, micronucleus organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9809^* Cite

Description

This section is cut at a level where the macronucleus is folded so it appears to be in 2 parts and where the micronucleus is visible. Contractile vacuole, perioral kinetids, and the peristome is evident. TEM taken on 6/10/69 by R. Allen with Philips 300 operating at 60kV. Neg. 3,570X. Bar = 2µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. A print of the negative was scanned and processed in Photoshop. This image is suitable for qualitative analysis. There is a high resolution version of this image in the library (CIL:39173) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Opercularia [NCBITaxon:168247]; Opercularia coarctata
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1657/1
Cellular Component: macronucleus; oral apparatus; mitochondrion; cell cortex; cytoplasm; contractile vacuole; micronucleus

Biological Context

Biological Process: macronucleus organization; cortical cytoskeleton organization; cytoplasm organization; contractile vacuole organization; micronucleus organization

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9809
Archival Resource Key (ARK): ark:/b7295/w9cil9809

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2789px	——
Y	2927px	——