CIL:9718, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Coleps hirtus; Cell Types:eukaryotic cell, , ; Cell Components:cilium axoneme, axonemal microtubule, axonemal dynein complex, plasma membrane, microtubule basal body, cytoskeleton, microtubule cytoskeleton, actin cytoskeleton, bacteroid-containing symbiosome; Biological process:ciliary or flagellar motility, microtubule cytoskeleton organization, , detection of symbiont;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9718^* Cite

Description

Longitudinal section of somatic cilium showing the axoneme, basal body, and attached fibrous and microtubular elements. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/24/69 by R. Allen with Philips 300 operating at 60kV. Neg. 18,500X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:2868) is available for quantitative analysis. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Coleps hirtus
Cell Type: eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component: cilium axoneme; axonemal microtubule; axonemal dynein complex; plasma membrane; microtubule basal body; cytoskeleton; microtubule cytoskeleton; actin cytoskeleton; bacteroid-containing symbiosome

Biological Context

Biological Process: ciliary or flagellar motility; microtubule cytoskeleton organization; microfibril organization; detection of symbiont
Molecular Function: structural molecule activity

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9718
Archival Resource Key (ARK): ark:/b7295/w9cil9718

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4800px	——
Y	4032px	——