CIL:9714, Coleps hirtus, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Coleps hirtus; Cell Types:eukaryotic cell, , ; Cell Components:macronucleus, nuclear pore, heterochromatin, nucleolus; Biological process:macronucleus organization, nuclear pore organization, chromatin organization, chromosome organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9714^* Cite

Description

High resolution image of the macronucleus of Coleps hirtus. Heterochromatin and nucleoli are seen as smaller and larger stained bodies, respectively, in the macronucleus. Many nuclear pores exist in the nuclear envelope.

Technical Details

The material was prepared by standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/23/69 by R. Allen with Philips 300 operating at 60kV. Neg. 7,650X. The raw film was scanned with a Nikon Coolscan 9000ED. This high resolution image is suitable for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Coleps hirtus
Cell Type: eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component: macronucleus; nuclear pore; heterochromatin; nucleolus

Biological Context

Biological Process: macronucleus organization; nuclear pore organization; chromatin organization; chromosome organization
Molecular Function: structural constituent of chromatin; structural constituent of nuclear pore

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9714
Archival Resource Key (ARK): ark:/b7295/w9cil9714

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity; differences in adsorption or binding of stain
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3000px	——
Y	2432px	——