CIL:9701, Coleps hirtus, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Coleps hirtus; Cell Types:, ; Cell Components:contractile vacuole pore, microtubule associated complex, plasma membrane, actin cytoskeleton; Biological process:water homeostasis, microtubule cytoskeleton organization, ;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9701^* Cite

Description

Oblique section of the Coleps contractile vacuole pore revealing the supporting microtubules and adjacent fibrous layer. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 3/4/69 by R. Allen with Philips 300. Neg. 25,100X. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:9255) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Coleps hirtus
Cell Type: Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component: contractile vacuole pore; microtubule associated complex; plasma membrane; actin cytoskeleton

Biological Context

Biological Process: water homeostasis; microtubule cytoskeleton organization; microfibril organization
Molecular Function: control of water regulation

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9701
Archival Resource Key (ARK): ark:/b7295/w9cil9701

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: film; print from negative was scanned for photoshop

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3000px	——
Y	2366px	——