CIL:9699, Coleps hirtus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Coleps hirtus; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:contractile vacuole, smooth endoplasmic reticulum membrane, mitochondrion, mitochondrial outer membrane, mitochondrial inner membrane, , ; Biological process:cellular water homeostasis, long-chain fatty acid metabolic process, oxidative phosphorylation;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9699^* Cite

Description

The contractile vacuole of Coleps hirtus. The filled CV is continuous with tubules of the smooth spongiome. The tubular nature of mitochondrial cristae is particularly evident in this micrograph. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 3/2/69 by R. Allen with Philips 300. Neg. 16,000X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:9252) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Coleps hirtus
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component: contractile vacuole; smooth endoplasmic reticulum membrane; mitochondrion; mitochondrial outer membrane; mitochondrial inner membrane; tubular cristae; cortical membranes

Biological Context

Biological Process: cellular water homeostasis; long-chain fatty acid metabolic process; oxidative phosphorylation
Molecular Function: water transmembrane transporter activity; water channel activity; mitochondrial structure

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9699
Archival Resource Key (ARK): ark:/b7295/w9cil9699

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; Print from negative scanned for Photoshop.
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3000px	——
Y	2410px	——