CIL:9512, Xenopus laevis. CIL. Dataset

NCBI Organism:Xenopus laevis; Cell Components:spindle microtubule, condensed chromosome; Biological process:meiotic metaphase II

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9512^* Cite

Description

Frog (Xenopus laevis) egg arrested in metaphase of mitosis. Note the tiny metaphase spindle on the top left of the egg. Eggs were fixed in 90% MeOH; 10% (H20 500mM EGTA) and stained for DNA with YoPro1 (green) and tubulin (red) with antibody B-5-1-2 directly labeled with Alexa 647. A single z slice was collected with a Zeiss LSM 710 using a 10 X 0.3NA objective. Lasers were 488 (DNA stain) and 633 (Tubulin stain).

Biological Sources

NCBI Organism Classification: Xenopus laevis
Cell Type: CL:0000254
Cellular Component: spindle microtubule; condensed chromosome

Biological Context

Biological Process: meiotic metaphase II

Attribution

Names: Martin Wuehr; Timothy J. Mitchison

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9512
Archival Resource Key (ARK): ark:/b7295/w9cil9512

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: single-spot confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: YO-PRO-1; Alexa Fluor 647; labeled primary antibody
Processing History: unprocessed raw data

Sample Preparation

Methods: methanol fixed tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3264px	0.4341µm
Y	3264px	0.4341µm

Channel	Wavelength
1	488, 633µm