CIL:9057, Nephrotoma suturalis, spermatocyte. CIL. Dataset

Cell Types:spermatocyte Cell Components:cytoskeleton, spindle, nuclear chromosome; Biological process:spindle assembly involved in male meiosis II

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:9057^* Cite

Description

The events of meiosis I in a living insect spermatocyte beginning at diakinesis through telophase to the near completion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 30 sec over 4 hr by a Dage-MTI C300 video camera with Scion AG-5 frame grabber. A movie prepared from this stack of images is included in this grouped set of images.

Biological Sources

NCBI Organism Classification: Nephrotoma suturalis
Cell Type: spermatocyte
Cellular Component: cytoskeleton; spindle; nuclear chromosome

Biological Context

Biological Process: spindle assembly involved in male meiosis II

Attribution

Names: Rudolf Oldenbourg; James R. LaFountain
Pubmed: 15385630
Pubmed: 12790452

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL9057
Archival Resource Key (ARK): ark:/b7295/w9cil9057

Grouping This image is part of a group.

Imaging

Image Type: recorded image; Processed image based on 5 recorded images
Image Mode: polarization microscopy
Parameters Imaged: UV/visible/IR photons
Source of Contrast: intrinsic birefringence
Visualization Methods: relative retardation (retardance) measurement
Processing History: 5-frame algorithm t

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	640px	87nm
Y	480px	87nm

Time	30 seconds	460