Alternate header for print version


Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:832*  Cite 
Description

Purified rabbit skeletal muscle myosin in 0.6M KCl was deposited onto mica flakes, quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 11 degrees and visualized in a JEOL 100CX2 transmission electron microscope at 100 kev. Images were recorded on Kodak 4489 film at 100,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED. Note the classic appearance of the individual molecules with two globular heads (myosin subfragment 1) and the long rod-like tail. RIGHT image of a stereo pair.

Biological Sources
NCBI Organism Classification
Oryctolagus cuniculus
Cell Type
skeletal muscle cell
Cellular Component
cytoskeleton
Biological Context
Biological Process
skeletal muscle contraction
Molecular Function
ATPase activity, coupled to movement of substances
Attribution
Names
Ip, Wallace
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL832
Archival Resource Key (ARK)
ark:/b7295/w9cil832
Grouping This image is part of a group.
Sample Preparation
Methods
unfixed tissue
freeze_fracture/freeze_etch
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 4163px 0.0002µm
Y 2790px 0.0002µm