This HeLa cell is expressing two fluorescent proteins, including EGFP-CENP-B, a fluorescent protein that marks kinetochores (seen as paired dots oriented parallel to the x-axis and attached to opposite spindle poles), and Venus-centrin, which marks the centrosomes (seen as single dots near the left and right edges of the movie) and was depleted of the mitotic kinesin, Kif18A. In control cells at this stage in mitosis, sister kinetochore pairs typically undergo oscillatory movements around the equator of the spindle. In this cell depleted of Kif18A, however, kinetochores are still bioriented and undergo oscillatory movements but fail to align at the spindle equator. Depletion of Kif18A leads to a two-fold increase in oscillation amplitude (from 1.2 microns to 2.3 microns). The increased oscillation amplitude can be explained by both a decrease in the rate of directional switches and an increase in the velocity of movement. As kinetochore movements are highly coordinated with the dynamics of kinetochore microtubules, we hypothesize that Kif18A may control kinetochore movements by regulating kinetochore microtubule dynamics. Note that the centrin dots are approximately 19 microns apart at the start of the movie. This movie is part of a group that captured first place at the 2007 ASCB Celldance contest.