Fluorescence recovery after photobleaching (FRAP)kinetics of MeCP2 were recorded using a mouse fibroblast cell line stably expressing MeCP2-GFP. Live cells were imaged using a Zeiss LSM 510 meta confocal microscope with fluorescence excitation at 488 nm and a 63x plan-apo oil lens. The image series has 2 pre-bleach shots, one immediately after bleaching a 1 micrometer diameter circle of pericentromeric heterochromatin, followed by 103 images at 2 sec intervals.
Spatial Axis | Image Size | Pixel Size |
---|---|---|
X | 512px | 0.05µm |
Y | 512px | 0.504µm |
Time | 2 seconds | 125 |
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