CIL:7750, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Nassula; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:oral apparatus, microtubule associated complex; Biological process:oral apparatus organization, microtubule bundle formation;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7750^* Cite

Description

A higher resolution cross section of the oral basket from Nassula aurea (C.N.R.S., Yvette, France) through its “waist”. Rods, rod lamellae, and the surrounding sheath are seen. Cytopharyngeal vesicles fill the basket lumen and are scattered outside the basket. TEM taken on 3/2/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 6,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Nassula
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
Cellular Component: oral apparatus; microtubule associated complex

Biological Context

Biological Process: oral apparatus organization; microtubule bundle formation

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL7750
Archival Resource Key (ARK): ark:/b7295/w9cil7750

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4748px	2.5nm
Y	5294px	2.5nm