CIL:7749, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Nassula; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:macronucleus, nucleolus, nucleolus organizer region; Biological process:macronucleus organization, nucleolus organization, nucleolus to nucleoplasm transport;

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7749^* Cite

Description

Transmission electron micrograph of section through the macronucleus of Nassula sp containing many darkly staining nucleoli.

Technical Details

TEM taken on 4/1/69 by R. Allen with Philips 300 operating at 60kV. Neg. 7,650X. Bar = 1µm. A print of the negative was scanned and processed in Photoshop. This image is suitable for quantitative analysis. A lower resolution image (CIL:11039) is available in the library for qualitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydration in alcohol and embedding in epoxy resin. Microtome section thickness approximately 75nm. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Nassula
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
Cellular Component: macronucleus; nucleolus; nucleolus organizer region

Biological Context

Biological Process: macronucleus organization; nucleolus organization; nucleolus to nucleoplasm transport

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL7749
Archival Resource Key (ARK): ark:/b7295/w9cil7749

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	5024px	2nm
Y	6206px	2nm