CIL:7744, Potorous tridactylus tridactylus, primary cell line cell. CIL. Dataset

NCBI Organism:Potorous tridactylus tridactylus; Cell Types:primary cell line cell Cell Components:Golgi apparatus, nucleus;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7744^* Cite

Description

Details of a cryofixed Ptk tissue culture cell showing Golgi apparatus, mitochondria and a segment of nucleus with nucleolus at right. 40 nm epon section prepared from plunge frozen,and freeze substituted specimen. Image recorded at 55,000x with a Philips CM10 TEM operated at 80KV.

Biological Sources

NCBI Organism Classification: Potorous tridactylus tridactylus
Cell Type: primary cell line cell
Cell Line: Ptk1 kidney epithelium
Cellular Component: Golgi apparatus; nucleus

Attribution

Names: Mary Morphew; J. Richard McIntosh; Mark Ladinsky

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL7744
Archival Resource Key (ARK): ark:/b7295/w9cil7744

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); film
Image Mode: illumination by electrons; detection of electrons
Parameters Imaged: electron density
Source of Contrast: electron-dense stain
Visualization Methods: uranyl salt
Processing History: unprocessed raw data

Sample Preparation

Methods: cryofixed tissue; freeze substitution; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4268px	0.27nm
Y	6192px	0.27nm