CIL:7743, Potorous tridactylus tridactylus, primary cell line cell. CIL. Dataset

NCBI Organism:Potorous tridactylus tridactylus; Cell Types:primary cell line cell Cell Components:Golgi apparatus Biological process:protein maturation

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7743^* Cite

Description

Details of the Golgi apparatus seen in 40 nm epon sections of Ptk tissue culture cells after plunge freezing,and freeze substitution. Image recorded at 28,500x with a Philips CM10 TEM operated at 80KV

Biological Sources

NCBI Organism Classification: Potorous tridactylus tridactylus
Cell Type: primary cell line cell
Cell Line: Ptk1 kidney epithelium
Cellular Component: Golgi apparatus

Biological Context

Biological Process: protein maturation
Molecular Function: sorting, modifying and packaging of macromolecules

Attribution

Names: Mary Morphew; J. Richard McIntosh; Mark Ladinsky

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL7743
Archival Resource Key (ARK): ark:/b7295/w9cil7743

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); film
Image Mode: illumination by electrons; detection of electrons
Parameters Imaged: electron density
Source of Contrast: electron-dense stain
Visualization Methods: uranyl salt
Processing History: unprocessed raw data

Sample Preparation

Methods: cryofixed tissue; freeze substitution; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3668px	0.7nm
Y	4388px	0.7nm