CIL:7666, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Nassula; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cell cortex, clathrin coat, ; Biological process:cortical cytoskeleton organization, microtubule cytoskeleton organization, clathrin coat assembly;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7666^* Cite

Description

Higher resolution image of the surface of Nassula aurea. Early endosomes and clathrin coated vesicles. One clathrin coated pit at the bottom of a parasomal sac is shown. TEM taken on 2/22/71 by M. Aihara with Hitachi HU11A operating at 50kV. Neg. 10,750X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Nassula
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
Cellular Component: cell cortex; clathrin coat; parasomal sac

Biological Context

Biological Process: cortical cytoskeleton organization; microtubule cytoskeleton organization; clathrin coat assembly

Attribution

Names: M. Aihara; Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL7666
Archival Resource Key (ARK): ark:/b7295/w9cil7666

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4677px	1.4nm
Y	4774px	1.4nm