CIL:7624, Nassula, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Nassula; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:cell cortex, microtubule basal body, cytoskeleton; Biological process:cytoskeleton organization, , ciliary or flagellar motility;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7624^* Cite

Description

High resolution image of Nassula sp. showing rows of cross-sectioned basal bodies at the cell surface. TEM taken on 3/4/69 by R. Allen with Philips 300 operating at 60kV. Neg. 16,000X. The raw film was scanned with a Nikon Coolscan 9000ED. This image is suitable for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Nassula
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, catalog # LB1650/1
Cellular Component: cell cortex; microtubule basal body; cytoskeleton

Biological Context

Biological Process: cytoskeleton organization; cytoskeleton membranes; ciliary or flagellar motility

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL7624
Archival Resource Key (ARK): ark:/b7295/w9cil7624

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: elastic scattering
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4674px	0.94nm
Y	6234px	0.94nm