CIL:7340, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Opercularia [NCBITaxon:168247], , ; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:oral apparatus, macronucleus, cytoplasm, cell cortex; Biological process:cortical cytoskeleton organization, , cytoplasm organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7340^* Cite

Description

A high resolution overview of Opercularia coarctata showing that cytostome divides the peristome from the cytopharynx. The cytopharynx starts where the alveoli end in the oral region. TEM taken on 6/13/69 by R. Allen with Philips 300 operating at 60kV. Neg. 5,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is suitable for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Opercularia [NCBITaxon:168247]; Opercularia coarctata
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1657/1
Cellular Component: oral apparatus; macronucleus; cytoplasm; cell cortex

Biological Context

Biological Process: cortical cytoskeleton organization; oral organization; cytoplasm organization

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL7340
Archival Resource Key (ARK): ark:/b7295/w9cil7340

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4548px	3nm
Y	6109px	3nm