CIL:7337, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protista, ciliated Protista. CIL. Dataset

NCBI Organism:Opercularia [NCBITaxon:168247], , ; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:micronucleus, macronucleus, nuclear pore; Biological process:micronucleus organization, macronucleus organization, nucleocytoplasmic transport, ;

Licensing

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CIL:7337^* Cite

Description

A high resolution image of the micronucleus and macronucleus of Opercularia coarctata. The nuclear envelope has numerous pores, at least as many per unit length as in the macronucleus. Some areas of the outer membrane of the micronucleus envelope bare ribosomes. TEM taken on 6/11/69 by R. Allen with Philips 300 operating at 60kV. Neg. 23,200X. The raw film was scanned with an Epson Perfection V750 Pro. this image is suitable for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Opercularia [NCBITaxon:168247]; Opercularia coarctata
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protista; ciliated Protista
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1657/1
Cellular Component: micronucleus; macronucleus; nuclear pore

Biological Context

Biological Process: micronucleus organization; macronucleus organization; nucleocytoplasmic transport; membrane bound ribosomes

Attribution

Names: Richard Allen

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL7337
Archival Resource Key (ARK): ark:/b7295/w9cil7337

Grouping This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film; unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3699px	0.86nm
Y	4490px	0.86nm