CIL:7329, Mus musculus, blood vessel endothelial cell, primary cell line cell. CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:blood vessel endothelial cell, primary cell line cell; Cell Components:myosin II complex Biological process:cell division, actomyosin contractile ring contraction;

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CIL:7329^* Cite

Description

Primary endothelial cells from transgenic mouse expressing myosin heavy chain 2A fused to mCherry (red), transfected with GFP-myosin (green) regulatory light chain expression vector and demonstrate the role of myosin in cell division. Cells are embedded in 3D collagen gels. mage Name: myosin cell division timelapse Biological Source: Primary mouse (mus musculus) aortic endothelial cell. 2D images over time. 0.1274 micron/pixel, 520x490 pixels, 20 seconds interval between timepoints.Time shown is in minutes:seconds. Images collected with a spinning disk confocal on a Nikon TE1000 with a 60X 1.2NA water objective using 400 ms exposure for mCherry and 200 ms for GFP. See Fischer et al for details regarding preparation. http://www.ncbi.nlm.nih.gov/pubmed/19185493

Biological Sources