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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:7249*  Cite 
Description

NG108-15 cells expressing EGFP-actin. NG108-15 cells are a cell line that is derived from a mouse neuroblastoma and a rat glioma. This is one of two images related images that show actin dynamics in the growth cones of the NG108 cells. This image shows a more detailed view of filopodial dynamics in the growth cone and the related image shows captures the actin dynamics of multiple processes on the NG108. Images were collected as a single z slice time series on a Carl Zeiss 5 LIVE microscope. The Zeiss 5 LIVE uses a cylindrical lense to create a sheet of illumination that is rapidly scanned in the vertical direction and detected on a linear CCD array to enable faster imaging. The images were collected with a 63X 1.4 NA plan apo objective. Filers: NFT 490 and BP 500-525. 17 micron slit width. Pixel dwell 1.94 ms yielded approximately 2 frames collected per s.

Biological Sources
NCBI Organism Classification
mouse/rat NG108-15
Cell Line
NG108-15
Cellular Component
growth cone
Biological Context
Biological Process
axon guidance
Molecular Function
actin binding
Attribution
Names
Galbraith, Catherine
Galbraith, James
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL7249
Archival Resource Key (ARK)
ark:/b7295/w9cil7249
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
slit-scan confocal
Parameters Imaged
fluorescence emission
Source of Contrast
UV/visible/IR photons
Visualization Methods
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
unfixed tissue
Relation To Intact Cell
living tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 331px 0.2083µm
Y 237px 0.2083µm
Time 0.5 seconds 129