CIL:709, Gallus gallus, nucleate erythrocyte. CIL. Dataset

NCBI Organism:Gallus gallus; Cell Types:nucleate erythrocyte Cell Components:chromatin Biological process:DNA packaging, chromosome organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:709^* Cite

Description

TEM image showing the basic nucleosomal organization of eukaryotic chromatin. Beaded chains of nucleosomes separated by linker DNA are seen emanating from a central mass of less dispersed chromatin.

Technical Details

Chicken erythrocyte nuclei were allowed to decondense in a low salt high pH buffer, fixed with HCOH, centrifuged onto a carbon coated EM grid, and stained with uranyl acetate. (Spreading technique of Oscar L Miller Jr).

Biological Sources

NCBI Organism Classification: Gallus gallus
Cell Type: nucleate erythrocyte
Cellular Component: chromatin

Biological Context

Biological Process: DNA packaging; chromosome organization
Molecular Function: structural constituent of chromatin

Attribution

Names: woodcock, chris

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL709
Archival Resource Key (ARK): ark:/b7295/w9cil709

Imaging

Image Type: film
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: visualization of contiguous regions; uranyl salt
Processing History: unprocessed raw data

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4096px	0.73nm
Y	2804px	0.73nm