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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:6269*  Cite 
Description

Rabbit psoas skeletal muscle fibers were blended in the presence of 5 mM MgATP to dissociate thick and thin filaments. The medium was then gently replaced with 0.3M KCl to dissociate thick filaments. Dissociated filaments were adsorbed onto mica flakes (Heuser JE, J Mol Biol. 1983 Sep 5;169(1):155-95), quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 11 degrees and visualized in a JEOL transmission electron microscope operated at 100 kev. This image was recorded on Kodak 4489 film at 80,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner. Stereo pair with CIL:6268.

Biological Sources
NCBI Organism Classification
Oryctolagus cuniculus
Cell Type
skeletal muscle cell
Cellular Component
cytoskeleton
Biological Context
Biological Process
skeletal muscle contraction
Molecular Function
ATPase activity, coupled to movement of substances
Attribution
Names
Ip, wallace
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL6269
Archival Resource Key (ARK)
ark:/b7295/w9cil6269
Grouping This image is part of a group.
Dimensions
Spatial Axis Image Size Pixel Size
X 4142px 0.00025µm
Y 2749px 0.00025µm