Live-cell imaging of NF-κB immune response in Drosophila S2* cells. Cells stimulated after 60 minutes show nuclear translocation of NF-κB and subsequent transcription of Dpt gene.
Cells were adhered to glass-bottom wells coated with concanavalin A (a lectin protein), and stimulated with 10 µg/mL DAP-type peptidoglycan at time point 5.
Microscopy Setup
Imaging was performed on a Nikon A1R point-scanning confocal microscope equipped with high-sensitivity GaAsP PMTs and a 60× oil immersion objective (NA = 1.4). Acquisition was controlled via Nikon Elements software.
Imaging Parameters
Time-lapse acquisition:
