Decellularized liver tissue re-seeded with HCT 116 cells. Fixed at day 3. Untreated.
BioTracker was illuminated with 638 nm and detected with a 660+ nm filter. Approximately 1.5 mW of 638 nm excitation power (laser output) was used to excite the BioTracker dye with an integration time of 20 ms per frame. Cells and the biomatrix scaffold were imaged simultaneously for each sample. Isotropic 1.793 µm3 voxels