FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
7.2_Test_Cell_GoldParticle_Segmentation is a multipage TIFF file illustrating the manually placed gold beads in a pattern as described in the Technical Validation section. Same as ‘GoldParticles.am’ file used for MATLAB volume analysis of this cell, but converted to TIFF to allow for online preview.
This file is a part of Dataset 7, comprised of files used as a technical validation dataset. It is an artificial dataset with manually placed gold beads, used to access the accuracy of the MATLAB script. The choice of gold bead placement location was unique for each row of stereocilia to control for potential errors of stereocilia placement within the rows during MATLAB analysis. No beads were placed on the surface of the kinocilium.
