CIL:50716 Maryna V. Ivanchenko, Marcelo Cicconet, Hoor Al Jandal, Xudong Wu, David P. Corey, Artur A. Indzhykulian (2019). FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles: 5.4_Cell_5_Stereocilia

NCBI Organism:Mus musculus, ; Cell Types:; Cell Components:, , , ; Biological process:;

Licensing

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Description

FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.

Technical Details

Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.

5.4_Cell_5_Stereocilia_Segmentation is a multipage TIFF file illustrating stereocilia segmentation results. Same as ‘Towers.am’ file used for MATLAB volume analysis of this cell, but converted to TIFF to allow for its online preview.

This file is a part of Dataset 5, comprised of files related to the Cell #5 of current study. The numbering order of the files reflects the order of operations performed during the analysis.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: Inner Ear Outer Hair Cell
Cellular Component: Hair Cell stereocilia; Cuticular plate; Mitochondria; Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1) labeling (gold beads)

Biological Context

Biological Process: Native auditory Hair Cells at postnatal day 4

Attribution

Names: Maryna V. Ivanchenko; Marcelo Cicconet; Hoor Al Jandal; Xudong Wu; David P. Corey (http://corey.med.harvard.edu/); Artur A. Indzhykulian (https://ailab.hms.harvard.edu/)
Link: Scientific Data article; Nature Communications article
OTHER: Massachusetts Eye and Ear; Harvard Medical School; 5.4_Cell_5_Stereocilia_Segmentation

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL50716
Archival Resource Key (ARK): ark:/b7295/w9cil50716

Grouping This image is part of a group.

Imaging

Image Mode: Serial Electron Microscopy; Focused Ion Beam Scanning Electron Microscopy (FIB-SEM)
Visualization Methods: Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid); Immunogold labeling with 10 nm gold beads