FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
3.3_Cell_3_Stack_Filtered is a multipage TIFF file (2.6×2.6×15 nm³ voxel size) filtered with mean shift filter (kernel: 3D, 3 > max distance X & Y: 3 > noise spread: 1.0), unsharp filter (unit: 8 > kernel: 3D, 3 > standard deviation: 1.0 > unsharp factor: 1.0) and histogram balancing filter (set to reference image #170).
This file is a part of Dataset 3, comprised of files related to the Cell #3 of current study. The numbering order of the files reflects the order of operations performed during the analysis.
