CIL:48104, Rattus norvegicus, skeletal muscle cell. CIL. Dataset

NCBI Organism:Rattus norvegicus; Cell Types:skeletal muscle cell Cell Components:actin filament, nucleus;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:48104^* Cite

Description

Cryopreserved rabbit skeletal muscle cells were revived and stained for actin (in red) to reveal cytoskeleton and DAPI (in blue) to label nuclei.

Technical Details

Rabbit skeletal muscle cells were fixed and immunostained for actin (from Santa Cruz), and DAPI (from Life Technologies). Images were acquired with a 40X (Plan Fluor, NA 0.75) lens, SPOT RT Slider CCD camera and SPOT Basic image capture software. Image generated with the Image J color combine function.

Biological Sources

NCBI Organism Classification: Rattus norvegicus
Cell Type: skeletal muscle cell
Cellular Component: actin filament; nucleus

Attribution

Names: Natalie Prigozhina

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL48104
Archival Resource Key (ARK): ark:/b7295/w9cil48104

Imaging

Image Type: charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody
Data Qualifiers: processed data

Sample Preparation

Methods: Dispersed cells cryopreserved; revived and cultured in vitro

Dimensions