CIL:48103, rabbit, skeletal muscle cell. CIL. Dataset

Cell Types:skeletal muscle cell Cell Components:cytoskeleton, nucleus;

Licensing

Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:48103^* Cite

Description

Cryopreserved rabbit skeletal muscle cells were revived and stained for actin (in red), desmin (in green) to reveal the intermediate filament essential for the structural integrity and function of muscle, and DAPI (in blue) to label nuclei.

Technical Details

Rabbit skeletal muscle cells were fixed and immunostained for actin (from Santa Cruz), desmin (from Sigma), and DAPI (from Life Technologies). Images were acquired with a 40X (Plan Fluor, NA 0.75) lens, SPOT RT Slider CCD camera and SPOT Basic image capture software. Image generated with the Image J color combine function.

Biological Sources

NCBI Organism Classification: rabbit
Cell Type: skeletal muscle cell
Cellular Component: cytoskeleton; nucleus

Attribution

Names: Natalie Prigozhina

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL48103
Archival Resource Key (ARK): ark:/b7295/w9cil48103

Imaging

Image Type: charge coupled device (CCD)
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody
Data Qualifiers: processed data

Dimensions