CIL:47559, Staphylococcus aureus, neutrophil, endothelial cell. CIL. Dataset

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:47559^* Cite

Description

Visualization of neutrophil recruitment in capillaries proximal to agarose beads. (Figure 3 - Mac1)

Technical Details

Mice were anesthetized with ketamine/xylazine, and then subcutaneous tissue on a dorsal flap was exteriorized. GFP staphylococcus aureus beads were inserted. Antibodies were injected into the vasculature to label neutrophils (red) and endothelial cells (blue). Blocking antibodies against the molecule Mac1 were administered after 2 hours of infection.

Biological Sources

NCBI Organism Classification: Staphylococcus aureus
Cell Type: neutrophil; endothelial cell

Biological Context

Biological Process

Attribution

Names: Mark Harding

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL47559
Archival Resource Key (ARK): ark:/b7295/w9cil47559

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: spinning disk confocal microscopy
Source of Contrast: antibody labelling
Processing History: Converted from volocity to .avi

Sample Preparation

Relation To Intact Cell: intact cells-in vivo imaging

Dimensions

Spatial Axis	Image Size	Pixel Size
X	——	512µm
Y	——	512µm