CIL:47308, Staphylococcus aureus, neutrophil, endothelial cell. CIL. Dataset

NCBI Organism:Staphylococcus aureus; Cell Types:neutrophil, endothelial cell; Biological process:cell death

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:47308^* Cite

Description

Visualization of neutrophil recruitment in capillaries proximal to agarose beads. (Figure 4 - Propidium iodide experiments)

Technical Details

Mice were anesthetized with ketamine/xylazine, and then subcutaneous tissue on a dorsal flap was exteriorized. GFP staphylococcus aureus beads were inserted. Antibodies were injected into the vasculature to label neutrophils (red) and endothelial cells (blue). Blocking antibodies against the molecule Mac1 were administered after 2 hours of infection.

Biological Sources

NCBI Organism Classification: Staphylococcus aureus
Cell Type: neutrophil; endothelial cell

Biological Context

Biological Process: cell death

Attribution

Names: Mark Harding

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL47308
Archival Resource Key (ARK): ark:/b7295/w9cil47308

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: spinning disk confocal microscopy
Source of Contrast: antibody labelling
Processing History: Converted from volocity to .avi

Sample Preparation

Relation To Intact Cell: intact cells-in vivo imaging

Dimensions

Spatial Axis	Image Size	Pixel Size
X	——	512µm
Y	——	512µm