CIL:45501, Homo sapiens, pluripotent stem cell. CIL. Dataset

NCBI Organism:Homo sapiens; Cell Types:pluripotent stem cell Biological process:neuron differentiation

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:45501^* Cite

Description

Differentiated human induced pluripotent stem cells (iPSCs) following differentiation protocol. Cell nuclei were stained with DAPI (blue), nestin (red) and beta3-tubulin/Tuj1 (green). Note the fate decision of some stem cells towards neuronal differentiation (left side) whereas the second type of cells (right side) differentiated towards brain microvascular endothelial cells (right side).

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: pluripotent stem cell

Biological Context

Biological Process: neuron differentiation

Attribution

Names: Abraham Al-Ahmad; Eric Shusta

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL45501
Archival Resource Key (ARK): ark:/b7295/w9cil45501

Imaging

Image Type: recorded image
Image Mode: fluorescence microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	600px	——
Y	800px	——