CIL:43851, Mus musculus, mammary epithelial cell. CIL. Dataset

NCBI Organism:Mus musculus; Cell Types:mammary epithelial cell Cell Components:nucleus, intermediate filament, cell-cell junction; Biological process:lactation

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:43851^* Cite

Description

The image shows a section of a developing wildtype mouse mammary gland immunostained for vimentin (red), beta-catenin (green) and DNA (blue). This is an example of a control image for experiments demonstrating the functions of the Elf5 protein. See: Chakrabarti et al. 2012. Elf5 inhibits the epithelial-mesenchymal transition in mammary gland development and breast cancer metastasis by transcriptionally repressing Snail2. Nat Cell Biol 14:1212-1222.

Technical Details

Mammary gland tissue from wildtype P17.5 mice was fixed in formaldehyde, embedded in wax, and sections immunostained for vimentin (red), beta-catenin (green), and DNA (DAPI, blue). Images were recorded using a Leica confocal microscope. See also: Chakrabarti et al. 2012. Elf5 inhibits the epithelial-mesenchymal transition in mammary gland development and breast cancer metastasis by transcriptionally repressing Snail2. Nat Cell Biol 14:1212-1222.

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: mammary epithelial cell
Cellular Component: nucleus; intermediate filament; cell-cell junction

Biological Context

Biological Process: lactation

Attribution

Names: Rumela Chakrabarti; Satrajit Sinha; Yibin Kang
Link: NIGMS Biomedical Beat

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL43851
Archival Resource Key (ARK): ark:/b7295/w9cil43851

Imaging

Image Type: recorded image
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: 4',6-diamidino-2-phenylindole (DAPI)

Sample Preparation

Methods: formaldehyde fixed tissue; tissue in wax embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	848px	——
Y	848px	——