CIL:41743, Bos sp., heart. CIL. Dataset

NCBI Organism:Bos sp.; Cell Components:mitochondrion, ; Biological process:aerobic respiration

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41743^* Cite

Description

Transmission electron micrograph of an unstained, unfixed cytochrome c oxidase crystal prepared from beef heart mitochondria imaged in the frozen hydrated state. The crystalline organization is seen within the ovoid structure in the lower portion of the image.

Technical Details

Samples of cytochrome c oxidase in suspension were applied to holey carbon films in a water saturated atmosphere, blotted, and plunged into a cryogen consisting of a mixture of 95% propane and 5% isopentane cooled by liquid nitrogen. Grids were examined using a Philips 420T TEM and images recorded at 46KX in the frozen hydrated state on Kodak SO-163 film. Negatives were scanned with a pixel size of 20x20 micrometers. See also: Frey TG and Murray JM 1994. Electron microscopy of cytochrome c oxidase crystals. J Mol Biol 237:275-297.

Biological Sources

NCBI Organism Classification: Bos sp.
Cell Type: heart
Cellular Component: mitochondrion; Cytochrome c oxidase

Biological Context

Biological Process: aerobic respiration
Molecular Function: electron transfer

Attribution

Names: John Murray; Terence Frey

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL41743
Archival Resource Key (ARK): ark:/b7295/w9cil41743

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: intrinsic mass distribution
Visualization Methods: visualization of contiguous regions
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: cryofixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3782px	0.43nm
Y	4636px	0.43nm