CIL:41740, Bos sp., heart. CIL. Dataset

NCBI Organism:Bos sp.; Cell Components:mitochondrion, ; Biological process:aerobic respiration

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41740^* Cite

Description

Transmission electron micrograph of an unstained, unfixed cytochrome c oxidase crystal prepared from beef heart mitochondria imaged in the frozen hydrated state. A crystalline organization is seen within the ovoid structure in the lower portion of the image.

Technical Details

Samples of cytochrome c oxidase in suspension were applied to holey carbon films in a water saturated atmosphere, blotted, and plunged into a cryogen consisting of a mixture of 95% propane and 5% isopentane cooled by liquid nitrogen. Grids were examined using a Philips 420T TEM and images recorded at 46KX in the frozen hydrated state on Kodak SO-163 film. Negatives were scanned with a pixel size of 20x20 micrometers. See also: Frey TG and Murray JM 1994. Electron microscopy of cytochrome c oxidase crystals. J Mol Biol 237:275-297.

Biological Sources

NCBI Organism Classification: Bos sp.
Cell Type: heart
Cellular Component: mitochondrion; Cytochrome c oxidase

Biological Context

Biological Process: aerobic respiration
Molecular Function: electron transfer

Attribution

Names: John Murray; Terence Frey

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL41740
Archival Resource Key (ARK): ark:/b7295/w9cil41740

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: intrinsic mass distribution
Visualization Methods: visualization of contiguous regions
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: cryofixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3800px	0.43nm
Y	4538px	0.43nm