CIL:41728, Cricetulus griseus, ovary. CIL. Dataset

NCBI Organism:Cricetulus griseus; Cell Components:nuclear chromosome Biological process:mitosis, mitotic metaphase, chromosome organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41728^* Cite

Description

High Voltage EM image of CHO metaphase chromosomes from a 'thick' section of metaphase-arrested cells treated with D20 to stabilize microtubules. This image was recorded with a specimen tilt of 45 degrees. The accompanying grouped image had a tilt of 55 degrees to create a stereo pair providing an oblique 3D view of the chromosomes.

Technical Details

CHO cells at metaphase were treated with 50% D2O, fixed with glutaraldehyde, embedded in epoxy resin and 0.5 micrometer 'thick' sections stained with uranyl acetate and lead citrate for HVEM observation at 1MeV. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources

NCBI Organism Classification: Cricetulus griseus
Cell Type: ovary
Cell Line: CHO
Cellular Component: nuclear chromosome

Biological Context

Biological Process: mitosis; mitotic metaphase; chromosome organization

Attribution

Names: Hans Ris

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL41728
Archival Resource Key (ARK): ark:/b7295/w9cil41728

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2824px	1nm
Y	4220px	1nm