CIL:41705, Cricetulus griseus, ovary. CIL. Dataset

NCBI Organism:Cricetulus griseus; Cell Components:nuclear chromosome Biological process:mitosis, mitotic metaphase, chromosome organization;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41705^* Cite

Description

High Voltage (1MeV) EM image of metaphase arrested CHO cell squashed in 3:1 acetic acid:ethanol and transferred to an EM grid. Portions of 4 pairs of chromatids are seen. This micrograph was captured at a specimen tilt of 55 degrees. The accompanying image has a tilt of 45 degrees, creating a stereo pair that provides an oblique stereo view of the chromatids.

Technical Details

Cells were metaphase arrested with colcemid, then exposed to a low ionic strength medium (0.07M phosphate buffer)containing 50% D20 as a microtubule stabilizer. Preparations were squashed in 3:1 acetic acid:ethanol,stained, and transferred to an EM grid. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources

NCBI Organism Classification: Cricetulus griseus
Cell Type: ovary
Cell Line: CHO
Cellular Component: nuclear chromosome

Biological Context

Biological Process: mitosis; mitotic metaphase; chromosome organization

Attribution

Names: Hans Ris

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL41705
Archival Resource Key (ARK): ark:/b7295/w9cil41705

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: squash

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4477px	1.27nm
Y	6624px	1.27nm