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NCBI Organism:Cricetulus griseus; Cell Types:epithelial cell Cell Components:nuclear chromosome, kinetochore; Biological process:chromosome organization, mitosis, mitotic metaphase;
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41581*  Cite 
Description

High voltage (1MeV) transmission electron microscopy image of a 0.5 micrometer section through a Chinese hamster ovary cell at metaphase showing a chromatid pair, with kinetochores at the centromere. The image was taken with a specimen tilt of 33 degrees and is one of a group of 5 tilts from 73 degrees to 33 degrees at 10 degree intervals. Pairs of images provide oblique stereo views of the chromosome.

Technical Details

CHO cells at metaphase were treated with 50% D2O, fixed with glutaraldehyde, embedded in epoxy resin and 0.5 micrometer 'thick' sections stained with uranyl acetate and lead citrate for HVEM observation. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources
NCBI Organism Classification
Cricetulus griseus
Cell Type
epithelial cell
Cell Line
CHO
Cellular Component
nuclear chromosome
kinetochore
Biological Context
Biological Process
chromosome organization
mitosis
mitotic metaphase
Attribution
Names
Hans Ris
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL41581
Archival Resource Key (ARK)
ark:/b7295/w9cil41581
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
lead salt
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in plastic embedment
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 2713px 1nm
Y 4210px 1nm