CIL:41454, Cricetulus griseus, epithelial cell. CIL. Dataset

NCBI Organism:Cricetulus griseus; Cell Types:epithelial cell Cell Components:nuclear chromosome, kinetochore; Biological process:chromosome organization, mitosis, mitotic metaphase;

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41454^* Cite

Description

High voltage (1MeV) transmission electron microscopy image of a 0.5 micrometer section through a Chinese hamster ovary cell at metaphase showing a chromatid pair, with kinetochores at the centromere. The image was taken with a specimen tilt of 62 degrees and is one of a group of 10 tilts from 77 degrees to 32 degrees at 5 degree intervals. Pairs of images provide oblique stereo views of the chromosome.

Technical Details

CHO cells at metaphase were fixed with paraformaldehyde followed by OsO4, embedded in epoxy resin and 0.5 micrometer 'thick' sections stained with uranly acetate for HVEM observation. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources

NCBI Organism Classification: Cricetulus griseus
Cell Type: epithelial cell
Cell Line: CHO
Cellular Component: nuclear chromosome; kinetochore

Biological Context

Biological Process: chromosome organization; mitosis; mitotic metaphase

Attribution

Names: Hans Ris

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL41454
Archival Resource Key (ARK): ark:/b7295/w9cil41454

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt; osmium tetroxide
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: formaldehyde fixed tissue; tissue in plastic embedment
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2824px	1nm
Y	4232px	1nm