CIL:41034, Rattus rattus, hepatocyte. CIL. Dataset

NCBI Organism:Rattus rattus; Cell Types:hepatocyte Cell Components:polysome, ribosome;

Licensing

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CIL:41034^* Cite

Description

Transmission electron micrograph of a polysome (polyribosome) purified from a rat liver homogenate by sucrose gradient centrifugation, and negatively stained with uranyl acetate. The linear arrangement of ribosomes bound to messenger RNA s clearly seen.

Technical Details

Samples were placed on carbon coated collodion films, negatively stained with aqueous uranyl acetate, and examined with a Philips 300 TEM. See also N. Yoshiaki et al. 1971. Structure of liver ribosomes studied by negative staining. J Mol Biol 60:303-323

Biological Sources

NCBI Organism Classification: Rattus rattus
Cell Type: hepatocyte
Cellular Component: polysome; ribosome

Biological Context

Biological Process

Attribution

Names: Yoshiaki Nonomura; Gunter Blobel; David Sabatini; George E. Palade
Published: N. Yoshiaki et al. 1971. Structure of liver ribosomes studied by negative staining. J Mol Biol 60:303-323
Pubmed: 4106575
Link: George E. Palade EM Slide Collection

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL41034
Archival Resource Key (ARK): ark:/b7295/w9cil41034

Grouping This image is part of a group.

Imaging

Image Type: film
Image Mode: transmission electron microscopy (TEM)
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Data Qualifiers: processed data

Sample Preparation

Methods: unfixed tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3197px	——
Y	3560px	——