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NCBI Organism:Cricetulus griseus; Cell Types:epithelial cell Cell Components:nuclear chromosome Biological process:chromosome organization, mitosis, mitotic metaphase;
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40704*  Cite 
Description

High voltage (1MeV) transmission electron microscopy image of a 0.5 micrometer section of a Chinese hamster ovary cell in metaphase containing a longitudinal section of a chromatid pair, and revealing the fiber-like structure. The image was taken with a specimen tilt of 45 degrees. Grouped with it is an image with a tilt of 55 degrees, providing a pair that affords an oblique stereo view of the chromosome.

Technical Details

Cells in metaphase were fixed, embedded in plastic, and 0.5 um sections cut and stained with uranyl acetate. See also: H. Ris 1981 Steroscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources
NCBI Organism Classification
Cricetulus griseus
Cell Type
epithelial cell
Cell Line
CHO
Cellular Component
nuclear chromosome
Biological Context
Biological Process
chromosome organization
mitosis
mitotic metaphase
Attribution
Names
Hans Ris
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40704
Archival Resource Key (ARK)
ark:/b7295/w9cil40704
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
chemically fixed tissue
tissue in plastic embedment
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 4792px 1nm
Y 6673px 1nm