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NCBI Organism:Mus musculus; Cell Types:fibroblast Cell Components:nuclear chromosome Biological process:chromosome organization, mitosis, mitotic metaphase;
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40689*  Cite 
Description

High voltage (1MeV) transmission electron microscopy image of an isolated metaphase chromatid pair from a mouse A9 fibroblast, showing the fiber-like structure. The image was taken with a specimen tilt of 55 degrees. Grouped with it is an image with a tilt of 45 degrees, providing a pair that affords an oblique stereo view of the chromosome.

Technical Details

Cells in metaphase were exposed to 75 mM KCl, spread on water, picked up on grids, stained with uranyl acetate and critical point dried. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
Cell Line
A9
Cellular Component
nuclear chromosome
Biological Context
Biological Process
chromosome organization
mitosis
mitotic metaphase
Attribution
Names
Hans Ris
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40689
Archival Resource Key (ARK)
ark:/b7295/w9cil40689
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
chemically fixed tissue
critical_point dried specimen
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 4063px 1.6nm
Y 5984px 1.6nm